Antibody Design/Humanization Service

The non-human antibodies entering the human body can cause severe rejection. For example, the entry of murine monoclonal antibodies into the human body will cause the human anti-mouse antibody response (HAMA response), which will affect the clinical application of antibodies and severely impair the safety and treatment effect. Antibody humanization is the process of genetic engineering and recombinant expression of animal-derived antibodies so that most or all of the antibody's sequence becomes human-derived, ensuring antibody affinity and specificity and minimizing the heterogeneity of antibodies to humans.

Figure 1. Antibody design. (Jun Zhan.;et al.2018)

Method

The current common computational antibody design method starts with the modeling of antibody and antibody-antigen complexes. Select antibody sequences for antigen binding experiments, for example, bind to soluble antigens or cell-expressed antigens, and further optimize the conjugate through affinity maturation methods. However, this type of method often requires modeling of the overall structure, which requires a large amount of calculations and takes a long time. Therefore, an antibody design based on the CDR of the specific binding site of the antibody and the antigen was proposed. This method simplifies the calculation and design of the antibody to the modeling and design of the antibody CDR, which greatly reduces the computational requirements.

Overall solutions

CD ComputaBio uses computer-aided structural simulation design to humanize mouse monoclonal antibodies, rabbit monoclonal antibodies, etc. Here are the content:

• Annotation of antibody sequence: annotation of antibody heavy and light chain variable regions, constant regions, and CDR regions, recognition of antibody Germline genes.
• Analysis of antibody sequence: prediction of antibody post-translational modification sites (such as glycosylation sites, etc.), antigen linear epitope prediction.
• Antibody structure prediction and optimization: prediction of full-length antibody, antibody Fab region, scFv structure.
• Antibody humanization design, immunogenicity prediction.
• Antibody-antigen interaction prediction: recognition of antigen conformational epitope, determination of hapten junction site.
• Design and modification of antibody molecules: antibody affinity maturation, antibody stability optimization, bispecific antibody design, antibody aggregation effect analysis.

Our services

Related Services:

• Structure-based Virtual Screening (SBVS) Service
• Reverse Virtual Screening
• 2D-QSAR Service
• 3D-QSAR Service

CD ComputaBio' antibody design/humanization service can significantly reduce the cost and labor of the subsequent experiments. Antibody design/humanization service is a personalized and customized innovative scientific research service. Each project needs to be evaluated before the corresponding analysis plan and price can be determined. If you want to know more about service prices or technical details, please feel free to contact us.

Reference:

1. Jun Zhan.;et al. In Silico Methods in Antibody Design. Antibodies.2018. 7(3), 22.

• Antibody Modeling Services
• Antigenic Determinant Prediction
• Antibody Affinity Maturation Service
• Computer-Aided Antibody Screening and Design
• Antibody Reformatting Service
• Antibody Characterization and Analysis