DNA Modification Analysis Service
DNA modifications play a crucial role in regulating gene expression and maintaining genomic stability. CD ComputaBio's DNA modification analysis service leverages advanced computational tools and expertise to analyze and interpret DNA modification data, enabling researchers to uncover insights into epigenetic regulation and its impact on biological processes and diseases. From data processing to functional annotation, our services provide a comprehensive solution for DNA modification analysis.
Introduction to DNA Modification
DNA modifications, such as DNA methylation and hydroxymethylation, are epigenetic changes that regulate gene expression without altering the underlying DNA sequence. These modifications are essential for normal development, cellular differentiation, and response to environmental cues. Dysregulation of DNA modifications is associated with various diseases, including cancer, neurological disorders, and metabolic syndromes. With the advent of high-throughput sequencing technologies, vast amounts of DNA modification data are generated, requiring sophisticated computational methods for analysis.
Figure 1. Computational Strategy for DNA Modification. (Balasundaram A, et al., 2022)
Methods for Modification Analysis
- Bisulfite Sequencing: A gold standard method for detecting DNA methylation by converting unmethylated cytosines to uracil.
- Chromatin Immunoprecipitation (ChIP): Used to analyze protein-DNA interactions and identify modified DNA regions.
- Mass Spectrometry: Provides precise quantification of DNA modifications at the molecular level.
Our Services
Employing bioinformatics approaches, machine learning, and statistical modeling enables the analysis of high-throughput sequencing data, the identification of patterns of modifications, and the correlation of these modifications with phenotypic outcomes.
By Types
- DNA Methylation Service: CD ComputaBio's DNA methylation service uses cutting-edge technology to accurately locate the methylation modification sites at the 5th carbon position of cytosine of CpG dinucleotides in the genome. We use technologies such as whole genome methylation sequencing (WGBS) and methylated DNA immunoprecipitation sequencing (MeDIP-seq) to comprehensively analyze the methylation map.
- DNA Acetylation Service: We focus on DNA acetylation research, focusing on the effects of histone acetylation on chromatin structure and gene activity. Through advanced experimental methods, we detect the acetylation state of histone lysine residues under the action of acetylase. Using high-resolution technology, we observe how chromatin becomes loose after acetylation modification and promotes gene transcription.
- DNA Phosphorylation Service: CD ComputaBio provides professional DNA phosphorylation services to accurately analyze the addition and regulation mechanism of phosphate groups on DNA molecules. By studying the regulation of DNA phosphorylation on gene expression, replication and transcription, we help customers in the fields of molecular biology and gene therapy.
- DNA Ubiquitination Service: DNA Ubiquitination Service focuses on revealing the regulation of ubiquitination modification on the function of DNA-related proteins. Through techniques such as immunoprecipitation, we identify the ubiquitination sites of proteins that interact with DNA.
By Workflow
Sample Extraction: Begin by isolating DNA from diverse samples like tissues or cells. Employ optimized kits or chemical methods to purify DNA, removing proteins and contaminants. Ensure high - quality DNA for subsequent analysis.
Detection: Use advanced techniques such as bisulfite sequencing for methylation, or specific antibodies for acetylation, etc. These methods precisely identify and quantify different DNA modifications at the molecular level.
Data Analysis: Bioinformatics tools are then utilized to process the detected data. Analyze modification patterns, identify differentially modified regions, and correlate them with gene expression data for biological insights.
Result Delivery: Finally, deliver comprehensive reports. Include detailed data on modification sites, levels, and their potential biological implications. Provide visualizations like graphs and tables for easy understanding.
Our Advantages
Advanced Computational Tools
State-of-the-art software and algorithms ensure accurate and reliable analysis of DNA modification data.
Expert Team
A team of computational biologists, bioinformaticians, and geneticists provides tailored solutions for each project.
Customized Approaches
Services are adapted to meet specific project requirements, from data processing to functional annotation.
CD ComputaBio's DNA modification analysis service offers a comprehensive suite of computational tools and expertise to accelerate discoveries in epigenetics. By leveraging advanced algorithms, data integration, and visualization tools, this service enables researchers to uncover insights into DNA modifications and their impact on gene regulation and disease. If you are interested in our services or have any questions, please feel free to contact us.
Reference:
- Balasundaram A, Doss C G P. unraveling the structural changes in the DNA-binding region of tumor protein p53 (TP53) upon hotspot mutation p53 Arg248 by comparative computational approach. International Journal of Molecular Sciences, 2022, 23(24): 15499.
* For Research Use Only.
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